Current protocols in immunology by John E Coligan; et al

By John E Coligan; et al

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Removal of the thymus after day 3 does not alter the influence of the thymus on the development of later T cell-dependent antibody or cellular immune responses. ) 60- or 100-mm Petri plate, sterile 1. Remove newborns from their mother and take them to a separate room for the operation. The noises made by the newborns during surgery agitate the mother and may cause the mother to attack them when they are returned. 2. Place newborns on clean gauze, 10 to 15 cm from the incandescent desk lamp. 3. Anesthetize by hypothermia.

Again grasp the thymus at a point below the other forceps. By alternately grasping and lifting the thymus at successively lower positions, remove the thymus from the chest cavity. Rotating the forceps slightly to the left and the right will also aid in bringing the thymic lobes out of the chest. The thymus has been successfully mobilized when both lobes are evident outside of the chest, and only thin translucent connective tissue remains attaching the underside of the thymus to the mouse. 12a. One pair of forceps will now be under the thymus and the thin connective tissue should be visible.

9. Using the cranial forceps, retract the strap muscles by gently inserting the closed forceps through the muscle layer and then allowing forceps to open. Insert the caudal forceps and open so that the strap muscles as well as the chest is held open. The lobes of the thymus should now be visualized as two thin white discs overlying the heart. Retraction of the strap muscles is not always necessary: the strap muscles attach at a point near the second rib, so retraction of a chest incision made beyond this point often moves the strap muscles aside.

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