By H. J. Brass (auth.), Shimshon Belkin (eds.)
In the summer time of 1999, a global team of specialists convened in Jerusalem, Israel, with a purpose to outline the main environmental demanding situations dealing with humanity on the sunrise of the hot millennium and - the place attainable - suggest methods of addressing them. virtually 50 chosen articles are gathered within the current ebook, which constitutes a outstanding interdisciplinary blend of cutting-edge technology with the most recent perspectives on environmental legislations, schooling, and foreign cooperation. when a tremendous fraction of the booklet is dedicated to water-related matters (water caliber tracking, water and wastewater therapy, water-based overseas cooperation, and more), different sections care for well timed themes with regards to pollution, biodiversity, conservation, and schooling. The publication is meant for environmental scientists, execs, and scholars of all disciplines.
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R. com Abstract. Present methods for the detection of micro-organisms in the environment are slow, inefficient and often unreliable. Alternative approaches which are reliable and rapid, enabling results to be obtained within one working day, are required. The development of molecular techniques, in particular in situ hybridisation offers the potential for rapid and specific assays. This paper describes the use of oligonucleotide probes targeted against the 16S and 18S ribosomal RNA molecules of Escherichia coli and Cryptosporidium parvum for rapid and specific detection.
The instrument was a ChemScan C or a Scan RDI (Chemunex), equipped with a 488-nm argon laser and photomultipliers sensitive to the green and amber regions of the light spectrum. A computer visualized all detected fluorescent events in a primary and, following software discrimination, a secondary scan map. The results displayed in the latter were visually confirmed using an epifluorescence microscope with a moving stage, connected to the laser scanner, to differentiate bacteria from other fluorescent events that possibly escaped the software discrimination process.
1999). 3. EFFECTS OF SYNTHETIC MATERIALS Biofilm formation on the wall of the pipes is caused by compounds present in the water and by compounds originating from the pipe material. In the Netherlands PVC is the main pipe material. , 1994). In this test the ATP concentration on pieces of material incubated in slow sand filtrate at 25°C is assessed as a function of time. Typical results are presented in Figure 2. E 'E 1000 iii o 100 200 300 250 500 o 250 500 Incubation time (days) Fig. 2. The biofilm concentration (pg ATP/cm2) on glass, unplasticized PVC (a), polypropylene (b), silicone tubing (c), as a function of time in the BFP test conducted at 25 DC.