Neurons: Methods and Applications for the Cell Biologist by Peter J. Hollenbeck, James R. Bamburg

By Peter J. Hollenbeck, James R. Bamburg

This ebook lays out a variety of easy strategies for growing to be and accomplishing experiments with many types of neurons. topics contain peripheral and important neurons from vertebrate and invertebrate assets, in addition to neuron-like mobilephone traces. It additionally explains fresh advances in our skill to introduce exogenous proteins and genes to neurons in tradition. systems for winning protein infiltration, biolistic transfection, electroporation, and viral transgenic equipment in neurons also are offered. * comprises tradition method for greater than a dozen forms of CNS and PNS neurons * comprises newest and trustworthy strategies from specialist practitioners for particular experimental purposes* Addresses the most recent recommendations for transfecting neurons

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And Strittmatter, S. M. (2000). Molecular basis of semaphorin-mediated axon guidance. J. Neurobiol. 44, 219–229. Overly, C. , and Hollenbeck, P. J. (1996). Dynamic organization of endocytic pathways in axons of cultured sympathetic neurons. J. Neurosci. 16, 6056–6064. Overly, C. , Rieff, H. , and Hollenbeck, P. J. (1996). Axonal and dendritic organelle transport in hippocampal neurons: Differences in organization and behavior. J. Cell Sci. 109, 971–980. , Liem, R. , Brady, S. , and Black, M. M.

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Pp. 177–205. MIT, Cambridge, MA. Johnson, M. I. (2001). Primary cultures of sympathetic ganglia. In ‘‘Protocols for Neural Cell Culture’’ (S. Fedoroff and A. , pp. 71–94. Humana Press, Totowa, NJ. Johnson, M. , and Argiro, V. (1983). Techniques in the tissue culture of rat sympathetic neurons. Methods Enzymol. 103, 334–347. Mahanthappa, N. , and Patterson, P. H. (1998). Culturing mammalian sympathoadrenal derivatives. In ‘‘Culturing Nerve Cells’’ (G. Banker and K. , pp. 289–307. MIT, Cambridge, MA.

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